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To construct this vector based on CMV-SP-SBP-EGFP-GPI 14 , a signal peptide of human IL2 from STR-KDEL_SBP-EGFP-GPI (gift from Franck Perez, Addgene plasmid # 65294), two copies of mClover3 from pKanCMV-mClover3-mRuby3 (gift from Michael Lin, Addgene plasmid # 74252), a 95-aa long linker from pEBP-Sec61b-mTagBFP2-LL-LOV2 14 , and synthetic oligonucleotides coding NIAAKDEL-STOP were assembled

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7D, Supplementary Fig