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We next asked whether reactive oxygen species (ROS) or subsequent NRF2 induction caused by GSH depletion could impact SLC25A39 levels

Pan SW, Yen YF, Kou YR, Chuang PH, Su VY, Feng JY, Chan YJ, Su WJ

The PLA results suggested that the reintroduction of UBE2m WT promoted NEDD4 and NEDD8 binding in UBE2mKO HK-2 cells

To explore the role of RRM2 in the PI3K/Akt signaling pathway, we examined the expression levels of PI3K pathway-related proteins including Akt, phosphorylated Akt (pAkt), PI3K kinase p85, PI3K kinase p110, and PTEN in TPC-1, RRM2 knockdown ( RRM2 -KD-TPC-1), and RRM2 overexpression ( RRM2 -OE-TPC-1) cells by Western blot