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In the late stages of pregnancy, slower glycine de novo synthesis limits the ability of adolescent mothers to maintain glycine flux and compromises fetal growth 22

The positional orientation of the amide group relative to the cationic headgroup also influences transfection efficiency, with classical amide configurations, where the amine is proximal to the polar head, achieving efficiencies comparable to Lipofectamine 2000 across various cell types, whereas reverse isomeric amide bonds reduce efficiency due to increased molecular rigidity from Coulombic repulsion [114]

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