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Vodicka P, Vodenkova S, Danesova N, et al

A study further revealed that Bifidobacterium activates the hepatic farnesoid X receptor (FXR) pathway, thereby downregulating lipogenic gene expression and reducing endotoxin translocation to the liver, suggesting an emerging direction for metabolic dysfunction-associated steatotic liver disease management

siRNA-mediated RNA Interference For RNA interference, cells in 6-well plates were transfected with designed siRNAs (an irrelevant sequence [control] siRNA), GSTP1 siRNA-1 and GSTP1 siRNA-2, each at 5 nmol/L, using Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) for 48 h according to the manufacturers instructions

These results confirm that decreases in intracellular GSH (and associated increased oxidative stress) worsen T2 inflammatory protein expression and secretion in HAECs, while increasing their susceptibility to ferroptotic death