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To assess cell viability of the neurospheres, cells were seeded after dissociation in 100 L PrimNeuS media on a Nunclon Sphera 96-Well U-Shaped-Bottom Microplate (Thermo Scientific)

The therapy is named for its developer, Max Gerson, a German physician who emigrated to the United States and started a medical practice in New York City in 1938.[1,2] The Gerson therapy is rooted in the belief that cancer is a disease of the whole organism, the tumor being only a symptom of a diseased body