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At 1 h after d-xylose administration, blood samples were collected from the anterior vena cava into heparinized vacuum tubes and centrifuged (2000 g for 15 min at 4 C) to obtain plasma 22

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Notably, prolotherapy (dextrose prolotherapy) protocols with multiple sessions may be more aligned with the theoretical regenerative mechanism of action, as fibroblast activation, collagen synthesis, and tissue remodeling are typically gradual processes requiring repeated stimulation over time