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Disinfect the rubber stopper with alcohol and allow complete evaporation before drawing bacteriostatic water

Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 mM L-glutamine, and 5mM cytosme-D-arabinofuranoside from Sigma added at 2 days in vitro

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This characteristic allows exploratory evaluation of oral administration, although current data indicate that oral bioavailability is presumed lower than parenteral routes