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de Ledinghen V, Lecesne R, Raymond JM, et al

Cells were cultured in basal medium (advanced DMEM/F12 with 10 mM HEPES, 1x Glutamax, and Penicillin/Streptomycin of 100 U/mL) supplemented with B27 (Invitrogen), 1 M N-acetylcysteine (Sigma-Aldrich), 10 nM gastrin (Sigma-Aldrich), 50 ng/mL mEGF (PeproTech), 50 ng/mL rhHGF (Bio-Techne R&D), 100 ng/mL FGF-10 (Peprotech), 1% R-spondin 3 conditioned medium, 10 mM nicotinamide (Sigma-Aldrich) and 10 M Rho-kinase inhibitors (AbMole)

Ingredientes Celulose microcristalina [1] , Glutatio reduzido (G-SH) [3] , Hidroxipropilmetilcelulose [2] Enchimento invlucro da cpsula Setria uma marca registada da Kyowa Hakko Bio Co., Ltd