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The details of isolation, purification, and structural elucidation of unknown-1 (mitragynine pseudoindoxyl) and its chemical synthesis are described in the Supporting Information

For the induction of recombinant proteins, the yeast cells grown in SD minimal medium without uracil at 28C for 1 days were transferred to 10 volumes of uracil-less SD medium containing 2% (w/v) galactose instead of glucose to activate the GAL1 promoter on pYES2, and cultured at 28C for 1 days

Separating them by time of day, or alternating nights, avoids the issue entirely while still letting you benefit from both mechanisms across a week