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Overexpression of HOXA4 and HOXA9 genes promotes selfrenewal and contributes to colon cancer stem cell overpopulation

RNA isolation, reverse transcription, and quantitative real-time PCR (qPCR) Total RNA was extracted using Trizol (Invitrogen, Waltham, MA, USA)

2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C

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