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Cortices were dissociated for 20 minutes with trypsin (Thermo Scientific) at room temperature on a rotator before trypsin inhibitor with DNase solution was added to quench the reaction

In case the participant had not reached at least the first sleep stage 30 min after the administration of the first drug, the second drug was chosen: the participants who received Dex as the first drug received Mel as the second drug, and those who received Mel as the first drug were given Dex (route of administration was again randomly chosen)

2022;10 doi: 10.3389/fchem.2022.942585

These ingredients work synergistically together to help recycle and replenish Glutathione levels in the body