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For IM injections, some protocols start with low doses of 200 mg weekly and gradually increase up to a maximum of 800-1000 mg weekly

Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)

Researchers conclude that this study outcome does not question the importance of getting enough of these vitamins during pregnancy but highlights concerns about the impact of a vitamin B12 overdose on offspring