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In order to test if the GHK and GHK-PEG that were synthesized were active in promoting fibroblast proliferation, NIH3T3 cells were cultured in 24 well plates and treated with GHK or GHK-PEG at 1, 5 and 10 nM for 24 h, 48 or 72 h

Testing methodology Identity confirmation by mass spectrometry, purity assay, appearance check, and moisture content

Researchers became interested because early studies showed that combining these peptides produced accelerated healing timelines and improved tissue quality outcomes compared to individual peptide applications