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Cross-immunity with Haemaphysalis longicornis glutathione S-transferase reduces an experimental Rhipicephalus ( Boophilus ) microplus infestation

For IL-1 treatment, cells were incubated in plain Dulbeccos modified Eagles medium (DMEM):F12 medium containing 6 mM glucose and 2 mM glutamine overnight followed by stimulation with IL-1 (10 ng/ml) or 0.1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) (vehicle control)

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Next generation sequencing In long-read sequencing, multiple nucleotide sequences can be read at once, without the prior need of DNA cleavage and amplification [13]