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To the 10.000 rpm supernatant containing microsomes and cytosol fractions, 0.2 volume of 0.1 M calcium chloride solution was added, and further centrifuged at 10,000 rpm for 10 minutes to precipitate the microsomes

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In patients with underlying systemic vasculitis, immunosuppressive medications (azathioprine, methotrexate, mycophenolate mofetil), biologics, or plasma exchange can be considered

Intravenous iron supplementation improves quality of life and exercise capacity in PAH patients, potentially resulting from enhanced oxygen transport in skeletal muscle [252, 254, 255]