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10.1210/en.2016-1130 Endocrinology 53 EllaK.Cspnyi-KmiR.KldiK

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27 Jan 2020 Funding The research was supported by the Shanghai Municipal Commission of Health and Family Planning, Shanghai Shenkang Hospital Development Center, Shanghai Municipal Education Commission, National Natural Science Foundation of China, Shanghai Committee of Science and Technology, Xinhua Hospital of Shanghai Jiao Tong University School of Medicine, National Human Genetic Resources Sharing Service Platform, the National Key Research and Development Program of China, 111 Project, the Shanghai Municipal Science and Technology Major Project, Guangdong Key Project, and ZJ Lab

The N-terminal conjugation of a protein transduction domain (PTD) to the synthetic peptides enhanced the in vitro transduction of SNARE peptides without any alteration of their inhibitory activities ( Materials and Methods Cloning, Expression, and Purification of Recombinant SNARE Proteins Total RNA was obtained from RBL-2H3 cells using TRIzol Reagent (#15596026, Invitrogen, Carlsbad, CA, USA) and served as a template for cDNA synthesis with M-MLV reverse transcriptase (M1705, Promega, Madison, WI, USA) according to the manufacturers protocol