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The adduct was further concentrated, and excess TRaQ-G ligand was removed by desalting with Zeba spin desalting columns (7k molecular weight cutoff, 0.5 ml)

Whether this could have any clinical benefit for patients, however, remained an open question

Overall 32.2% of subjects had LH at only one site, and 65.8% of patients had LH at several site levels, primarily the abdomen (52.4%), followed by thighs (23.2%), arms (19.9%), and other unusual areas (4.5%), including the thigh area located immediately distal to the crural region, the areas immediately above the knee, and the proximal arm region (34.2%)

Weight loss typically begins in this phase, averaging 1 to 3 pounds per week for most patients, though individual results vary significantly based on starting weight, diet quality, and activity level