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Description

Paula FM, Leite NC, Vanzela EC, Kurauti MA, Freitas-Dias R, Carneiro EM, Boschero AC, Zoppi CC

The reaction was initiated by adding reaction mixture containing 40 mM HEPES buffer, pH 7.6, 2 mM ATP, 10.6 mM MgCl 2 , 200 mM KCl, 20 M FeCl 3 , 5 mM DTT and 0.3 mM [ 3 H]CDP (5548 cpm/nmol) in a final volume of 50 l