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2018;6:289291.e2

Poly(ethyleneglycol)- -maleimide- -NHS (NHS-PEG-VS) is added to this solution and incubated at room temperature while mixing

The TetraGFP-NLS protein showed an exclusive nuclear localization, whereas TetraGFP-NLS-NES1 and TetraGFP-NLS-NES2 displayed both cytoplasmic and nuclear signals, with a stronger cytoplasmic signal for the NES2 construct (Fig