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After washing three times with ND96 (96 mM NaCl, 2 mM KCl, 1 mM MgCl 2 , 1.8 mM CaCl 2 , and 5 mM HEPES-NaOH, pH 7.4), efflux was initiated by addition of 0.5 mM glutamine to the ND96 incubation buffer

When ROS are produced excessively, they can directly cause double-strand DNA breaks and induce apoptosis, or provoke depolarization of the mitochondrial membrane potential (Fig

The procedure was administered with a high degree of tolerability, did not induce dependency, and exhibited both stress-relieving and tranquilizing properties

Argentina midfielder Leandro Paredes physically tangled with Spain players Eric Garcia and Gavi, and Spain captain Rodri was hit in the stomach as he ran past Argentina defender Nahuel Molina