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The expression of genes was confirmed by qRT, PCR technique using SYBR Green dye

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The absorption of TMA from the gut is increased when permeability of the intestinal barrier is higher [20]

This amino acid supports relaxation and balanced alertness by increasing GABAknown as the brains calming neurotransmitterand promoting alpha brain wave activity[16 Trusted Source 2011 - Journal of Functional Foods Effects of l-theanine on attention and reaction time response ] , [17 Trusted Source 2017 - Canadian Journal of Physiology and Pharmacology Caffeine and theanine exert opposite effects on attention under emotional arousal ] , [18 Trusted Source 2022 - Cleveland Clinic Gamma-Aminobutyric Acid (GABA) ] , [19 Trusted Source 2019 - Psychology Today 3 Amazing Benefits of GABA ] , [20 Trusted Source 2008 - Asia Pacific Journal of Clinical Nutrition L-theanine, a natural constituent in tea, and its effect on mental state ]