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Fine line and wrinkle reduction occurs through multiple mechanisms, including increased dermal volume from enhanced collagen production, improved skin hydration, and better cellular organization

Some peptides act locally in tissue or trigger downstream signaling that outlasts measurable blood levels BPC-157 is a commonly cited example

doi: 10.1038/s41467-019-11718-4

HEK293 cells transfected with HA-tagged WT ghrelin receptor or mutants were seeded at a density of 4 10 4 cells per well into 96-well culture plates and incubated for 24 h at 37 C in 5% CO 2 .The cells were then incubated with 2 mol/L Fluo-4 AM in HBSS (5.4 mmol/L KCl, 0.3 mmol/L Na 2 HPO 4 , 0.4 mmol/L KH 2 PO 4 , 4.2 mmol/L NaHCO 3 , 1.3 mmol/L CaCl 2 , 0.5 mmol/L MgCl 2 , 0.6 mmol/L MgSO 4 , 137 mmol/L NaCl, 5.6 mmol/L D-glucose and 250 mol/L sulfinpyrazone, pH 7.4) at 37 C for 40 min