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Cell viability assays Cells were seeded in 96-well plates and treated with serial dilutions of DIMATE (0.01100 M) with or without PCN (40 M), BSO (100 M), or BCNU (50 M) for 12, 48, and 72 h and analyzed using alamarBlue dye (Thermo Fisher, Saint Herblain, France)

If inflammation and mitochondrial dysfunction are limiting factors in a patient's metabolic rate, glutathione supplementation may provide marginal benefit