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To check the effect of selenium, sodium selenite (Sigma-Aldrich, USA) in the concentration from 1 pM to 1 nM was supplemented to the medium for 3 days starting from the 5th day of differentiation

Cells lacking PCBP1 or PCBP2 exhibit loss of DOHH activity, as measured by the accumulation of partially modified deoxyhypusine and reduced levels of fully modified hypusine (20% of control) in living cells