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The fold change methylation levels of each CG locus were compared between groups using an unpaired t-test

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One of the most commonly reported side effects of metformin is gastrointestinal disorders, including reduced vitamin B 12 absorption

So, the preclinical and clinical studies showed that vitamin B12 is an essential micronutrient involved in the preservation of pain inhibitory and excitatory neurotransmitters balance, inflammation moderation, and consequently in the diverse behavioral process including sleep, learning, memory, and sensation of pain [7, 19]