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Matsuo et al, 2023)

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Recently, Slc12a8 was identified as specific NMN transporter ( NAD Biosynthesis: the Enzymatic Functions of NAMPT and NAPRT NAD turnover within the cell is dynamic, displaying circadian oscillations that are regulated by the core clock machinery CLOCK:BMAL1 ( de novo biosynthetic pathway, the PreissHandler pathway, and the salvage pathway, as reviewed in Houtkooper et al

Targeted CRISPR-Cas9 screens under compartment-specific GSH limitation To investigate compartment-specific vulnerabilities in cells with uncoupled mitochondrial and cytosolic GSH pools, we performed a metabolism-targeted CRISPR-Cas9 screen in Jurkat cells expressing GshF or mito-GshF in which the recently identified mitochondrial GSH transporter, SLC25A39, was knocked out (Fig