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Two microliters of each sample was injected onto a Accucore 150-AmideHILIC 100mm x 2.1mm column held at 25C, and low molecular weight thiols were separated by a gradient of 0.1% v/v formic acid (Buffer A) and acetonitrile (Buffer B): a linear gradient from 9085% B over 6 minutes followed by 85% B for 2 minutes, 60% B for 1.5 minutes, and 90% B for 2.5 minutes

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However, in diabetic individuals, this immune response is often compromised because of hyperglycemia, which impairs neutrophil function, including phagocytosis and migration, leading to ineffective pathogen clearance [19]

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