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Briefly, in a 200 l reaction volume, 5 g ml 1 recombinant GSTO1 in reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA and 1 mM DTT) was incubated with DMSO or different concentrations of inhibitors for 30 min at 37 C

The establishment of this theoretical framework not only provides a novel, cell-death-centered molecular perspective for a deeper understanding of the long-standing sex differences in atherosclerosis but also offers new insights for re-examining and interpreting the complex and often contradictory results of past hormone replacement therapy clinical trials

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doi:10.1177/1933719115623645 29