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A 24 h time point was chosen based on a previous report of using this time period for transfecting or treating 3T3-L1 cells with NNMT anti-sense oligonucleotides or a small molecule NNMT product inhibitor (1-MNA), respectively, for phenotypic measures

PMCID: PMC3296793

The 0.9% concentration is the established pharmaceutical standard balancing antimicrobial efficacy with compatibility across the range of pharmaceutical and research compounds reconstituted in Bacteriostatic Water