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doi: 10.1038/s41467-020-19193-y 206 KurotakiDKawaseWSasakiHNakabayashiJNishiyamaAMorseHC3rdet al

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Dosing frequency Once daily: 200-300 mcg, typically before bed Simplest protocol Aligns with natural nighttime GH surge Good for anti-aging and general wellness Twice daily (preferred for most goals): 100-150 mcg morning (fasted or post-workout) 100-150 mcg before bed Better mimics natural pulsatile release Superior for muscle growth and fat loss Three times daily (advanced): Used by some athletes and bodybuilders Morning, post-workout, and bedtime 100 mcg per dose Requires more commitment Timing considerations Best times to inject: Before bed: Aligns with natural GH peak during sleep

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