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Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKine TM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex /xMAP technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA)

For example, overexpression of the BRCP/ABCG2 transporter is associated with resistance to several anti-cancer drugs including doxorubicin, mitoxantrone and topotecan [65], whilst ABCB1/MDR1 is frequently overexpressed in drug-resistant cell lines and is found to correlate with poor overall survival in patients [20, 66]

The residual liquid from tubes was cleared by filtration and lyophilized to obtain crude gastric juice concentrate

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