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Some studies showed that secreted soluble Klotho levels decrease before FGF23 levels increase (33,34) but the sequence of events may differ depending on experimental models and diverse clinical conditions (35)

store in sealed containers with additional desiccant if needed Stability data: 12+ months at -20C, 24+ months at -80C for optimal activity retention Record storage date, conditions, and any temperature excursions in laboratory records pH and Buffer Considerations: Both peptides stable at physiological pH (7.0-7.4) Phosphate buffered saline (PBS, pH 7.4) suitable for most applications HEPES buffer (10-25 mM, pH 7.2-7.4) alternative for CO-independent systems Avoid extreme pH (9.0) which may cause degradation or aggregation For long-term storage, slightly acidic pH (6.0-6.5) may enhance stability for some peptides Stability Considerations: Both peptides carry modifications that improve protease stability (semaglutide via a DPP-4-resistant Aib8 substitution

100 g/mL) for another 24 h

[DOI] [PubMed] [Google Scholar] Seidel P, Roth M, Ge Q