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Real-time qPCR was performed using GoTaq qPCR Master Mix (Promega, A6002) in a QuantStudio 6 Flex thermocycler (Applied Biosystem) or 7900HT Fast Real-Time PCR System (Thermo Fisher)

Unlike HGH, which has a broad spectrum of effects on growth and metabolism, AOD 9604 specifically targets fat metabolism by mimicking the fat-burning effects of HGH without influencing blood sugar levels or promoting muscle growth