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This is not uncommon in those who have undergone rapid withdrawal without adequate explanation, often in hospital or detoxification centres but sometimes at home when their doctor has withdrawn prescriptions

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Stress : Chronic stress can alter gut microbiota composition and increase gut permeability, affecting the gut-brain communication

Cell lines and culture All cells were cultured in DME/F12 (50:50) mixed medium supplemented with 10% fetal calf serum, 2 mM glutamine, 1 mM pyruvate, 100 U ml 1 penicillin and 100 g ml 1 streptomycin at 37 C in a humidified incubator under 5% CO 2