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References to metabolic or adipose-related pathways are included for scientific context only and do not imply therapeutic or clinical outcomes

An untreated vitamin B12 deficiency can lead to neurological problems or pernicious anaemia, which occurs when your body doesn't have enough B12 to produce the amount of red blood cells it needs

doi: 10.3389/fphar.2020.583422 Received 16 July 2020 Accepted 29 September 2020 Published 24 November 2020 Volume 11 - 2020 Edited by Luca Antonioli, University of Pisa, Italy Reviewed by Nathalie Stakenborg, KU Leuven, Belgium Oksana Zayachkivska, Danylo Halytsky Lviv National Medical University, Ukraine Updates Copyright 2020 Mazzotta, Villalobos-Hernandez, FIORDA-DIAZ, Harzman and Christofi

After defrosting, the samples were centrifuged (1000 rpm, 4C), and the pellet was suspended in cold PBS and mixed with 0.6% low-melting point agarose