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Systematic review with meta-analysis: coffee consumption and the risk of gallstone disease

Briefly, frozen liver aliquots were homogenized in Serine Assay Buffer in a Potter-Elvehjem homogenizer and centrifuged at 15000 g, for 10 min at 4C

The citric acid cycle involves three NAD+-dependent dehydrogenase reactions: isocitrate dehydrogenase, -ketoglutarate dehydrogenase, and malate dehydrogenase, each generating NADH while releasing carbon dioxide and regenerating oxaloacetate for continued cycle operation