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Activity was initiated with the addition of 1 mM GSSG and 100 M NADPH and initial rates of NADPH oxidation were monitored at 340 nm

Following the rate determining formation of 8-enolate , this intermediate shows only marginal stabilization over the corresponding transition state and evolves to 8-B4 in a barrierless step

P.et al (2022a)

Physiol Behav 100:525534 Wakahara S, Konoshita T, Mizuno S, Motomura M, Aoyama C, Makino Y, Kato N, Koni I, Miyamori I (2007) Synergistic expression of angiotensin-converting enzyme (ace) and ace2 in human renal tissue and confounding effects of hypertension on the ace to ace2 ratio