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Oxidative stress, viral infection, heat shock, nutrient deprivation, and alkylating agents all lead to activation of the cap-independent translation through IRES thus increasing the amount of Nrf2 [41, 42]

Int J Cancer 149(7):14951511 Ye Z, Hu Q, Zhuo Q, Zhu Y, Fan G, Liu M et al (2020) Abrogation of ARF6 promotes RSL3-induced ferroptosis and mitigates gemcitabine resistance in pancreatic cancer cells

Lancet 370 , 511520 (2007)

After centrifugation, methanol-containing proteins were precipitated with cold trichloroacetic acid (TCA) and reduced with 500 mM sodium borohydride in 0.2 M borate buffer (pH 9.2) after overnight incubation at room temperature