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doi: 10.1186/s12933-025-02614-x 14 WenJLiLYangYOuDYangJXieJet al

The MCH bacterial group showed a significant decrease compared to the control (74.03 1.270 Vs 108.6 2.452, P = 0.0072**), also the 1st group showed a significant decrease compared with the control (61.57 2.194 Vs 108.6 2.452, P = 0.0097**), the 2nd injection group showed nonsignificant increased compared to control (160.4 13.35 Vs 108.6 2.452, P = 0.0686), also the Vacc + Bac showed non-significant decreased (68.87 10.35 Vs 108.6 2.452, P = 0.0638), the 1st injection showed non-significant decreased comparing with bacteria (61.57 2.194 Vs 74.03 1.270, P = 0.0581), while the 2nd injection showed significantly increased compared with a bacterial group (160.4 13.35 Vs 74.03 1.270, P = 0.0198*), furthermore the Vacc + Bac showed non-markedly decreased comparing with a bacterial group (68.87 10.35 Vs 74.03 1.270, P = 0.8478) (Fig