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IFN also induces an increase in barrier permeability through the reduction of ZO-1 and occludin expression in an adenosine monophosphate-activated protein kinase (AMPK)-dependent pathway, irrespective of the cellular energy levels 62

Davico C, Secci I, Vendrametto V, Vitiello B

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Borreliacidal assay Bb , strain B31, was seeded at 1 10 5 spirochetes/mL in 96-well round-bottom plates with a 1:10 dilution of heat-inactivated mouse sera, 1:10 guinea pig complement sera (Sigma S1639), 100 g/mL of LA-2 antibody (Absolute Antibody, Ab01070-3.0) (positive control), and diluted in BSK-H complete media for a total of 100 L